Fig. 7

Ectopic expression of LcGPX6 in Arabidopsis causes defects in seed development. A Ectopic expression levels of LcGPX6 in wild type Col and different transgenic lines. qRT-PCR was performed and the Ubiquitin 10 from Arabidopsis was used as the internal reference to analyze the relative level of gene expression. B NBT was used to detect the superoxide and H₂O₂ accumulation in the inflorescences of Col and 35S:LcGPX6-3 transgenic plants. Scale bars are 1000 μm. C DCF fluorescence images of silique, pisitil and stamen between the Col and 35S:LcGPX6-3 transgenic plants. Scale bars are 100 μm. D Phenotype of the siliques and seeds in wild type Col and 35S:LcGPX6-3 transgenic plants. Scale bars are 100 μm. E Quantitative assay of seed number per silique in wild type Col and LcGPX6 transgenic lines. Error bar indicates mean ± SD (n = 10 siliques). Statistical significance was determined via employing Independent-Sample t-test (***P < 0.001)