Fig. 7

BcRAE and BcFAT induce resistance in N. benthamiana. A and C Phenotypes of plant resistance to B. cinerea induced by transiently expressed proteins. BcFAT, BcRAE A and their site-mutated proteins C were transiently expressed in N. benthamiana, and the Agrobacterium harboring the empty PVX vector was used as the control. The treated tobacco was kept in the culture room for three days, followed by inoculation of B. cinerea spores resuspended in PDB of different dilutions on the Agrobacterium-infiltrated site. Protein expression was detected by WB, and Ponceau S staining served as the loading control. The inoculated plants were kept in a humid chamber at 22 °C. Photographs were taken 60 h after inoculation. Each assay contained at least nine leaves, and the experiment was repeated three times. B and D Statistics of colony diameters. Colony diameters of B. cinerea grown on BcFAT, BcRAE B and their site-mutated proteins D pretreated tobacco were measured 60 h after inoculation. Data from three independent experiments are presented as the mean ± SD. Asterisks represent a significant difference (Tukey test, *, P < 0.05, **, P < 0.01, ***, P < 0.001); E N. benthamiana leaves were infiltrated with 50 μM of control protein, BcFAT, and BcRAE. After 24 h of growth in the culture room, the treated leaves were picked for RNA extraction. qRT-PCR was used to analyze the expression levels of NbPR1a, NbPR1b, NbPTI5, and NbRBOHb. Nbef1α was used as the reference gene. Data from three independent experiments are presented as the mean ± SD. Asterisks represent a significant difference. (Tukey test, P < 0.05)