Fig. 4

BcRAE and BcFAT induced cell death in plants. A Protein sequence alignment of BcRAE and BcFAT conserved motifs. Identical residues are highlighted with a yellow background. Red letters marked with blue triangles indicate the conserved residues Ser, Gly, Asn, and His in the SGNH hydrolases. The numbers indicate the position of the amino acid in the protein sequence. B Cell death symptoms of tobacco caused by BcRAE or BcFAT. Tobacco leaves were infiltrated with 50 μM BcRAE or BcFAT. Photos were taken 3 d after infiltration. Black dashed circles indicate infiltrated sites. C Effect of protein concentration on the cell death-inducing activities of BcRAE and BcFAT. For each treated leaf, the protein-infiltrated area was similar in size. Symptoms were photographed 5 d after infiltration. D Effect of BcRAE and BcFAT on tomato and oilseed rape leaves. Tomato and oilseed rape leaves were infiltrated with 50 μM proteins. Images were taken 5 d after infiltration