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Fig. 2 | Molecular Horticulture

Fig. 2

From: Characterization of two SGNH family cell death-inducing proteins from the horticulturally important fungal pathogen Botrytis cinerea based on the optimized prokaryotic expression system

Fig. 2

Comparison of different protein expression systems. A Flow chart of the protein expression processes of different systems. B The reported CDIPs BcIEB1, BcSPL1, and BcXYG1 were used to test the screening effects of different expression systems. Agrobacterium tumefaciens for transient expression was infiltrated into tobacco leaves at OD600 = 0.5. Photos were taken five days after infiltration. Western blotting (WB) was performed to verify the expression of proteins in plants. SDS-PAGE verified the proteins expressed by the eukaryotic and prokaryotic expression systems. Proteins expressed by the eukaryotic and prokaryotic expression systems were infiltrated into tobacco leaves at a concentration of 50 μM. Photos were taken 3 d after infiltration. Empty vectors were used to express control proteins (CK), respectively

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Molecular Horticulture

ISSN: 2730-9401

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