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Fig. 1 | Molecular Horticulture

Fig. 1

From: Characterization of two SGNH family cell death-inducing proteins from the horticulturally important fungal pathogen Botrytis cinerea based on the optimized prokaryotic expression system

Fig. 1

Optimization of the prokaryotic expression system. A Expression of soluble recombinant cell death-inducing proteins (CDIPs) utilizing the optimized prokaryotic expression system. The reported CDIPs BcIEB1 (BCIN_15g00100), BcNEP1 (BCIN_06g06720), BcSPL1 (BCIN_03g00500), and BcXYG1 (BCIN_03g03630) were recombinantly expressed with trigger factor (TF) using the prokaryotic expression system, and the TF was expressed as a control. The supernatant of disrupted cells was used for protein purification. B The impact of TF on plants. TF at different concentrations were infiltrated into tobacco leaves, and 10 mM phosphate-buffered saline (PBS) was used as the negative control. Photos were taken 5 d after infiltration. Black dashed circles indicate infiltrated sites

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Molecular Horticulture

ISSN: 2730-9401

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