Fig. 7

TLR3 is involved in the biosynthesis of cuticle in A. annua. a–l Young leaves of WT (a), TLR3-OE (d), TLR3-RNAi (g) and TLR3-Cas9 (j) plants treated with toluidine blue (TB); mature leaves of WT (b), TLR3-OE (e), TLR3-RNAi (h) and TLR3-Cas9 (k) plants treated with TB; and leaf veins of WT (c), TLR3-OE (f), TLR3-RNAi (i) and TLR3-Cas9 (l) plants were treated with TB. Red arrows point to sites that took up toluidine blue. m Hierarchical clustering analysis of genes involved in biosynthesis of lipids in WT A. annua and TLR3-OE leaves. The color scale at the top represents the log-transformed reads per kilobase per million mapped reads