Fig. 2

TLR3 expression level and TLR3 subcellular localization in A. annua. a Relative TLR3 expression level in callus (stages S1–S3) and leaves 0–7 (L1–L7). S1, callus primary stage; S2, callus middle stage; S3, shooting stage. b Relative TLR3 expression level in roots, stems, old leaves, young leaves and flowers. c Schematic of the TLR3-YFP construct, driven by the 35S promoter. YFP, yellow fluorescent protein; LB, left border; RB, right border; T, termination sequence. d TLR3 is localized in the nucleus in Nicotiana benthamiana leaf epidermal cells. Free YFP (35S:YFP) was used as a control. e Schematic of the TLR3pro:GUS reporter construct. GUS, ß-glucuronidase. f–k GUS staining of A. annua callus and tissues from TLR3pro:GUS transgenic materials. Scale bars, 1 mm