Fig. 1
Transcriptome sequencing and differential gene analysis of leaves 0, 3, 5 and 7 of A. annua plants. a Leaves from a three-month-old plant, numbered from top (youngest) to bottom (oldest). b Glandular trichomes on leaf 0, leaf 3, leaf 5 and leaf 7 of A. annua visualized by fluorescence microscopy. Autofluorescence of glandular trichomes was captured with λex = 480 nm and λem = 535 nm. c Trichome density in A. annua leaf 0, leaf 3, leaf 5 and leaf 7. Asterisks indicate significant differences relative to leaf 0 (Student’s t-test; **, P < 0.01). Data are means ± standard error (SE). d Clustering analysis of 2,123 genes in different leaves. The x axis represents leave samples from four developmental stages (leaf 0, leaf 3, leaf 5 and leaf 7); the y axis represents gene expression. Twenty-five clusters were identified. e Venn diagram showing the extent of overlap between differentially expressed genes in the comparisons leaf 0 vs leaf 3; leaf 3 vs leaf 5; and leaf 5 vs leaf 7. f Maximum likelihood phylogenetic tree reconstructed based on six identified A. annua transcription factors and 131 Arabidopsis thaliana transcription factors