Fig. 1

A Probe extracts RNA of I. sanguinea. B Probe of different specifications. C Evaluation of the SPGE method by PCR of Actin-7. M: DNA Marker 2000; CK1: negative control of SPGE technology; 1: Extraction of RNA by SPGE technology, reverse transcription. PCR product; CK2: negative control of improved CTAB method; 2: Extraction of RNA by improved CTAB method, reverse transcription, PCR product contrast. D Influence of different specification probes on the extraction quality of RNA, M: DNA Marker 2000; CK: negative control; 1: product of 0.14*6 mm probe; 2: product of 0.22*6 mm probe; 3: product of 0.16*7 mm probe; 4: product of 0.25*13 mm probe. E Influence of different culture time on the extraction quality of RNA. M: DNA Marker 2000; CK: negative control; 1: product of 12 h culture time; 2: product of 8 h culture time; 3: product of 4 h culture time; 4: product of 2 h culture time. F Influence of different embedding time on the extraction quality of RNA. M: DNA Marker 2000; CK: negative control; 1: product of 6 h embedding time; 2: product of 4 h embedding time; 3: product of 2 h embedding time; 4: product of 1 h embedding time. G Influence of different concentrations of Oligo dt25 on the extraction quality of RNA. M: DNA Marker 2000; CK: negative control; 1: product of 1uM Oligo dt25; 2: product of 3uM Oligo dt25; 3: product of 5uM Oligo dt25. H Influence of storage conditions on the storage time of the probe. M: DNA Marker 2000; CK: negative control; 1: product of 0d storage time; 2: product of 7d storage time; 3: product of 14d storage time; 4: product of 21d storage time; 5: product of 28d storage time. I A simplified gene extraction technique for RNA extraction in I. sanguinea