Fig. 5

Functional validation of PbrWRKY62 involved in fruit putrescine biosynthesis and thus chilling resistance. a Subcellular localization of PbrWRKY62. 4’,6-diamidino-2-phenylindole (DAPI) was used as a nuclear indicator (Kapuscinski 1995). b Impact of transient genetic transformation in the ripe ‘Dangshansuli’ fruit on putrescine abundance. (b-i) Transient overexpression of PbrWRKY62 gene. The ripe ‘Dangshansuli’ fruit transformed with the empty vector was used as a control. (b-ii) Transient silence of PbrWRKY62 gene. The ripe ‘Dangshansuli’ fruit co-transformed with empty pTRV2 and pTRV1 was used as a control. The expression level of PbrWRKY62 in control fruit was set as 1.0 for RT-qPCR. c Impact of overexpressing PbrWRKY62 gene in tomato on fruit putrescine biosynthesis and thus chilling resistance. (c-i) Visual quality change. (c-ii) Putrescine content and chilling injury index. Tomato fruits at 35 DAFB, including the wide-type (control) and overexpressing (OE) lines, were harvested and then exposed to 4 ℃ for 10 d followed by 20 ℃ storage for 7 d. Data represented the mean value of three biological replicates, and different lowercase letters meant significance between samples (p < 0.05)