Fig. 3

Overexpression of RcMYB8 improved tolerance to salinity stress. A Phenotypes of VC and pSuper:RcMYB8 under salinity stress. Performance of VC and pSuper:RcMYB8 rose seedlings under normal and salinity stress conditions. Nine-week-old rose plants were subjected to 0 and 200 mM NaCl for 3 d. Scale bar: 1 cm. B Relative expression of RcMYB8 in VC and pSuper:RcMYB8. RcUBI2 was used as an internal control of three biological replicates. C and D Electrolyte leakage (C) and chlorophyll content (D) of leaves in VC and pSuper:RcMYB8 under salinity stress. E and F The epidermis of VC and pSuper:RcMYB8 leaves under a fluorescence microscope, with blue–white spots representing the callosum. Scale bar: 100 μm. G Phenotypes of VC and pSuper:RcMYB8 rose calli treated with 200 mM NaCl for 15 days. Scale bar: 1 cm. H PCR analysis of pSuper:RcMYB8 lines using genomic DNA. +, positive control using the plasmid as template; VC: empty vector transformed plant used as negative control; #1, #2, and #3, pSuper:RcMYB8 lines. M: 2 kb DNA ladder. I Fresh weight in VC and pSuper:RcMYB8 under salinity stress. J NBT and DAB staining of callus tissue after salt stress treatment. Scale bar: 1 cm. Data represent the mean ± SD (n = 3)