Fig. 5

Self S-RNase reduces the expression levels of PbCOB.A.1, PbCOB.A.2, and PbC2H2.K16.2. a The expression patterns of PbABF.E.2, PbCOB.A.1, and PbCOB.A.2 in the pollen tubes treated with non-self S-RNase, self S-RNase, and buffer (control). b Phylogenetic classification of C2H2-type ZFP genes in pear, strawberry, papaya, orange, peach, apple, and grape. A1 to A15 are the clusters in group A; B, C, D, E, F, H, L, M, N, R, S, T, X, and Y indicate the corresponding groups; G1 to G4 are the clusters in group G; I1 to I19 are the clusters in the group I; J1 and J2 are the clusters in group J; K1 to K19 are the clusters in group K; O1 and O2 are the clusters in group O; P1 and P2 are the clusters in group P; Q1 and Q2 are the clusters in group Q; U1 to U4 are the clusters in group U; V1 and V2 are the clusters in group V; W1 to W3 are the clusters in group W; Z1 and Z2 are the clusters in group Z. The number above or below the line is the bootstrap value. The accession numbers were listed in Table S2. c The left panel showing the differential expression analysis of nine C2H2-type ZFP genes in the self-pollinated styles of ‘Dangshansuli’ (self-pollination) and in the ‘Dangshansuli’ styles pollinated with ‘Huanghua’ pollen (cross-pollination). The right panel showing the qRT-PCR analysis of nine C2H2-type ZFP genes in the pollen tubes treated with non-self S-RNase, self S-RNase, and buffer (control). Standard error was calculated from three replicates. Analysis of variance was calculated by Student’s t-test. Lowercase letters (a and b) indicate P < 0.05