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Fig. 2 | Molecular Horticulture

Fig. 2

From: Self S-RNase reduces the expression of two pollen-specific COBRA genes to inhibit pollen tube growth in pear

Fig. 2

The decreased expression of PbCOB.A.1 and PbCOB.A.2 inhibits pollen tube growth. a Subcellular localization of PbCOB.A.1- and PbCOB.A.2-GFP fusion proteins. The white line represent a scale (10 μm). FM4-64 is a dye that forces the plasm membrane to present red fluorescence, specifically. b The expression level of PbCOB.A.1 and PbCOB.A.2 was tested by qRT-PCR in pollen tubes with AS-ODN, S-ODN, or buffer (control) treatment. c The length of pollen tubes with AS-ODN, S-ODN, or buffer (control) treatment. Standard errors were calculated from three replicates for qRT-PCR analysis and from at least 90 pollen tubes for pollen tube growth analysis. Analysis of variance was calculated by Student’s t-test. Lowercase letters (a and b) indicate P < 0.05

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Molecular Horticulture

ISSN: 2730-9401

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