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Fig. 6 | Molecular Horticulture

Fig. 6

From: Multiomics analysis provides new insights into the regulatory mechanism of carotenoid biosynthesis in yellow peach peel

Fig. 6

Transcripts of PpMYB9 are targeted by mdm-miR858. A RNA secondary structure analysis predicted the stem-loop structure formed by the mdm-miR858 precursor (pre-mdm-miR858). The mature mdm-miR858 sequence is shown in red. B The mdm-miR858 precursor sequence were inserted into the pICH86988 vector to form an effector, and the PpMYB9 target site (MYB9TS) and its mutant (MYB9mTS) fused with a GFP reporter gene were inserted into the pICH86988 vector to form a reporter plasmid. C Site analysis of mdm-miR858 targeting shear PpMYB9. MYB9mTS (negative control) is a mutant of MYB9TS. D Effect of mdm-miR858 on PpMYB9 activity. The coexpression of pre-mdm-miR858 with MYB9TS did not exhibit GFP signaling, whereas substitution of the MYB9mTS did not affect GFP expression

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Molecular Horticulture

ISSN: 2730-9401

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