Fig. 4

Evaluation of the effect of crRNA positions on editing efficiencies during multiplex genome editing. A Mutation frequencies mediated by LbCas12a in 41B cells using the TMTs-crRNA array. Bars are mean of 3 technical replicates from one experiment. Error bars are SD. DR, direct repeat. B Percentage of edited plants regenerated from 41B cells in A. Chi, chimera; WT, wild type; He, heterozygotes; Bia, biallele mutants. C Mutation frequencies mediated by LbCas12a in 41B cells using the reverse TMTs-crRNA array. The positions of crRNAs in the array were altered by exchanging the order of crRNAs. D Mutation frequencies mediated by LbCas12a in 41B cells using the reverse PDS1-TMTs-crRNA array. Data are shown as means ± SD from three technical replicates