Fig. 1

Efficient and transgene-free genome editing in banana using the gene-deletion system. A Strategies for generating transgene-free genome-edited plants and illustration of constructs for genome editing. Primer sets for detecting transgene-free mutants are indicated with orange arrows. B Procedure for transgene-free genome editing in banana. C Results of tests for transgene-free mutants using six primer sets in 10 representative mutant plants. M: DNA marker; V: vector plasmid; R: Kan + ; WT: wild type; /: non-determined; + : GUS staining positive; -: GUS staining negative; UBQ: ubiquitin gene. D Genome-edited examples of genotyping results for five mutants. E Gene-editing efficiencies achieved when MaPDS (JQ762260.1) was mutated in the T0 generation. F Mapping of construct DNA in the banana genome using whole genome resequencing data