Fig. 4

Overexpression of LlC3H18 causes growth defectives. A Detection of LlC3H18-overexpression lines by RT-PCR. The 5-day-old seedlings were used to detect the expression of LlC3H18 in transgenic Arabidopsis lines. PCR of the endogenous control and test gene was performed with 28 and 30 cycles, respectively. AtActin2 was used as an endogenous control. B Seedlings of wild-type and transgenic lines grown on MS medium for 3 weeks. Scale bar = 1 cm. C Rosette radii of the plants which grown on MS medium for 3 weeks were counted. Bars are means ± SD of the tested plants (n = 9). D The 10-days-old seedlings were transferred from agar plates to soil for two weeks. The representative picture based on three replicates. Scale bar = 1 cm. E Rosette radii of the plants which grown on the soil for two weeks were counted. Bars are means ± SD of the tested plants (n = 9). F The 10-days-old seedlings were transferred from agar plates to soil for three weeks. Scale bar = 1 cm. The representative picture based on three replicates. G Bolting time for wild-type and transgenic lines. Bars are means ± SD of three independent experiments (n = 9, Student’s t-test, *P < 0.05). H The number of rosettes of the bolting transgenic and wild-type plants. Bars are means ± SD of three independent experiments (n = 9, Student’s t-test, *P < 0.05)