Fig. 2

Subcellular localization assay of LlC3H18. A Detection of fluorescence signals in tobacco leaf cells co-transfected with GFP-LlC3H18, the nuclear marker RFP-NLS, the PB marker RFP-AtDCP2, the SG marker RFP-AtPABP8 at room temperature (RT, 22°C) and under HS (37°C, 3 h). Scale bar = 50 µm. B Detection of fluorescence signals in tobacco leaf cells co-transfected with GFP-LlC3H18, and the nuclear marker RFP-NLS at room temperature (RT, 22°C), under HS (37°C, 3 h), and after recovery 1 h from HS (HS + R, 22°C). Scale bar = 50 µm. C RNA-EMSA assay of GST- LlC3H18 protein and ARE sequence. One representative image based on three independent experiments. D GFP-ARE or GFP-MutG co-transformed with LlC3H18. GFP-MuG (replace the A residue in ARE with G) as a negative control. RT, room temperature, 22°C; HS, heat stress, 37°C, 3 h. Scale bar = 50 µm. E The GFP intensity in (D) is measured. Data are presented as the mean ± SD of three replicates, with different letters indicating statistically significant difference (Student–Newman–Keuls test, P < 0.05)