Fig. 3
Evaluation of Citrus Huanglongbing (HLB) resistance in CaLasSDE460 transgenic plants under 25 °C and 32 °C treatment for three months. A and B Healthy wild-type (WT) and transgenic plants before CaLas-infected. C Quantitative analysis of CaLas contents in WT and transgenic plants under 25 °C and 32 °C treatment for one month. D Quantitative analysis of CaLas contents in WT and transgenic plants under 25 °C and 32 °C treatment for two months. E Quantitative analysis of CaLas contents in WT and transgenic plants under 25 °C and 32 °C treatment for three months. F HLB symptoms in the transgenic plants and WT controls under 25 °C treatment for three months. G HLB symptoms in the transgenic plants and WT controls under 32 °C treatment for three months. The bacterial populations (CaLas cells µg.−1 of citrus DNA) were determined using qPCR. (H) Statistic analysis of symptoms in the leaves under 25 °C and 32 °C treatment for three months. Percentage (%) of symptomatic leaves per plant was calculated by the number of symptomatic leaves out of the total number of leaves. Bars represent the average ± standard error of the means from three plants per lines(n = 3). WT, wild type; OE-#, transgenic plants. Different letters at the top of the bars indicates significant differences from the WT control (p < 0.05, Student’s t-test)