Fig. 8
Terpene production and AcTPS gene expression in tissue-cultured leaves and young fruit after hormone treatment. A Terpene accumulation in tissue-cultured leaves of kiwifruit ‘Hort16A’ and ‘Hayward’ after MeJA (JA) treatment. Leaf tissues were ground to powder and terpenes analysed by SPME GC–MS. B Terpene emission from tissue-cultured leaves of kiwifruit after MeJA treatment. Terpenes were collected by Tenax TA for 5 d and analysed by GC–MS. C Expression of all full-length AcTPS genes affected by MeJA treatment in tissue-cultured leaves. Gene expression was determined by qRT-PCR. D Terpenes accumulating in the tissue of 45 d old Red5 fruit after treatment with MeJA, SA and SA + MeJA. Fruit tissues were ground to powder and terpenes analysed by SPME GC–MS Compounds detected in fruit at > 100 ng∙g−1 are shown. E Expression of all full-length AcTPS genes affected by MeJA treatment of young fruit. Gene expression was determined by qRT-PCR. Data are means ± SE (n = 3). The Student’s t-test was used to test for statistical significance. Asterisks indicate a significant difference (*P < 0.05, **P < 0.01) between MeJA treated and untreated samples