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Fig. 6 | Molecular Horticulture

Fig. 6

From: The terpene synthase (TPS) gene family in kiwifruit shows high functional redundancy and a subset of TPS likely fulfil overlapping functions in fruit flavour, floral bouquet and defence

Fig. 6

Volatile terpenes produced by over-expression of AcTPS genes in E. coli. Recombinant enzymes were purified by Ni2+ affinity and gel filtration chromatography. Terpene volatiles were collected by SPME and analysed by GC–MS in triplicate. A AcLIS/NES + GDP as substrate; B AcLIS/NES + FDP; C AcNES3 + FDP; D AcGES + GDP; E AcNES2 + FDP; F AcBCS + FDP. The X-axis represents the retention time of the peak outflow and the Y-axis represents the relative intensity of the chromatographic peak. The lower panel shows the mass-to-charge ratios (m/z). The concentrations of all terpene volatiles measured are detailed in Table S5

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