Fig. 4

CmBBX7 regulates CmFTL1 directly. Interactions of CmBBX7 protein with the promoters of CmFTL1 in the yeast one-hybrid assay. SD/-T/-H/-L indicates Trp, His, and Leu synthetic dropout medium, respectively. The 3-AT concentration is 60 mM for CmFTL1pro. b Effector and reporter vector construction diagrams for the dual-luciferase assays. c Transient expression in N. benthamiana leaves of the pCmFTL1::LUC transgene. Graph showing luminescence intensity. d The ratio of LUC to REN activity. Error bars indicate the SD for six biological replicates. Significant differences are indicated by asterisks (** p < 0.01, ANOVA, Tukey’s correction). e–f EMSA of CmBBX7 binding to the CORE and TG-box elements of CmFTL1 promoter. ‘ + ’ indicates presence and ‘ − ’ indicates absence. g ChIP-qPCR of the enrichment of DNA fragments CORE (–95 to –91 bp) and TG-box (–762 to –757 bp) in the CmFTL1 promoter. Error bars indicate the SD for six biological replicates. Significant differences are indicated by asterisks (** p < 0.01, ANOVA, Tukey’s correction).