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Fig. 1 | Molecular Horticulture

Fig. 1

From: The NAC transcription factor MdNAC4 positively regulates nitrogen deficiency-induced leaf senescence by enhancing ABA biosynthesis in apple

Fig. 1

Response of MdNAC4 to ABA treatment. a Expression levels of MdNAC4 in apple seedling leaves treated with different phytohormones. Apple seedlings grown in the nutrient bowl were treated with 50 μM abscisic acid (ABA), 50 μM methyl jasmonate (MeJA), and 50 μM 1-aminocyclopropane-1-carboxylic acid (ACC) for 9 h by adding solutions containing ABA, ACC, and MEJA to the nutrient bowl. b Expression patterns of MdNAC4 after 50 μM ABA treatment. Samples were taken at designated times (0, 1, 3, 6, 9, 12 and 15 h) for RNA extraction. c Expression levels of senescence-related genes (MdNYC1, MdPAO, MdSAG12, MdSAG29 and MdSAG39) after 50 μM ABA treatment for 9 h. (d) GUS staining and qRT–PCR analysis of MdNAC4 promoter transgenic apple calli. Control: no treatment; ABA treatment: 50 μM ABA treatment for 9 h. The expression level in untreated samples was set at 1. Error bars indicate the SDs of the three technical replicates and three biological replicates. Asterisks indicate significant differences between two independent samples according to t tests (*, P < 0.05 and **, P < 0.01)

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Molecular Horticulture

ISSN: 2730-9401

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