Fig. 5

Cuticle-embedded polysaccharides are altered in the shn2 mutant. A Sugar content; the inset table represents the sugar composition expressed as total sugar mole percentage. The degree of methyl esterification (DM) refers to the molar amount of methanol per 100 mol of galacturonic acid while the degree of acetylation (DA) is reported as the weight of acetic acid/weight of polysaccharides. Values are mean ± SD (n = 3). Values in bold indicate significant difference from the WT. a, P < 0.01; b, P < 0.05 (Student’s t test). Rha, rhamnose; Ara, arabinose; Xyl, xylose; Man, mannose; Gal, galactose; GalA, galacturonic acid; Glc, glucose. B Polysaccharide composition. Cellulose: glucose deduced from second hydrolysis; hemicellulose: total neutral sugar with glucose from second hydrolysis only; pectin: galacturonic acid. Values are mean ± SD (n = 3)