Fig. 6
From: Yang cycle enzyme DEP1: its moonlighting functions in PSI and ROS production during leaf senescence
MdDEP1 triggers ROS overproduction in apple leaf cells. A. ATPase content in the WT and MdDEP1 transgenic apple plantlets. B. Protoplasts isolated from WT and MdDEP1-overexpressing apple leaves were incubated in C-H2DCFDA for 90 s. C. The fluorescence signals of protoplasts in (B) were quantified by pixel intensity. Data represent means ± SD of 12 to 15 individual protoplasts. D. DAB staining for H2O2 in the leaves of the WT and MdDEP1 transgenic apple plantlets. E. DAB staining intensity as determined with imageJ software. F. NBT staining for superoxide in the leaves of the WT and MdDEP1 transgenic apple plantlets. G. NBT staining intensity as determined with imageJ software. Note: In (A), (C), (E), and (G), the data are shown as the mean ± SE, which were analyzed based on more than 9 replicates. Statistical significance was determined using Student’s t-test in different samples. *P < 0.01; **P < 0.001