Fig. 3

RhNAP- or RhCKX6-silencing delays senescence in rose petal discs. RhNAP and RhCKX6 expression was silenced in petal discs by virus induced gene silencing (VIGS). A The phenotypes of the petal discs were recorded and photographed at various time points (days) until necrosis was observed. The ion leakage (B) and relative expression of RhSAG12 by qRT-PCR (C) were determined. n = 3 ± standard deviation values are shown in (B) and (C); Student’s t-test, *P < 0.05