The terpene synthase (TPS) gene family in kiwifruit shows high functional redundancy and a subset of TPS likely fulfil overlapping functions in fruit flavour, floral bouquet and defence

Table 2 Kinetic properties of recombinant TPS enzymes

	K_m (μM)	V_max (nM∙min⁻¹)	K_cat (s⁻¹)	K_cat/K_m (s⁻¹∙mM⁻¹)
AcBCS
FDP	1.54 ± 0.54	13.00 ± 3.10	0.0087 ± 0.0020	5.87 ± 1.10
AcNES2
FDP	5.55 ± 0.73	0.30 ± 0.03	1.89E-05 ± 2.07E-06	3.48E-03 ± 8.73E-04
AcLIS/NES
GDP	30.95 ± 3.81	3.38 ± 0.22	0.00021 ± 0.0001	0.00678 ± 0.0011
AcLIS/NES
FDP	7.43 ± 1.25	4.33 ± 0.12	0.00082 ± 0.0002	0.1103 ± 0.025
AcNES3
FDP	84.69 ± 18.46	1.80 ± 0.01	0.00026 ± 0.0001	0.00307 ± 0.00046
AcGES
GDP	0.76 ± 0.21	3.36 ± 0.23	0.00143 ± 0.0003	1.88 ± 0.65

Kinetic parameters of purified recombinant enzymes were determined in 50 mM Bis–Tris propane buffer pH 7.5. Parameters for FDP (0–50 μM) were obtained in the presence of 10 mM MgCl₂. All values represent mean ± SE, n = 3. K_m, Michaelis constant; V_max, maximum velocity; K_cat, turnover

ISSN: 2730-9401