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Fig. 7 | Molecular Horticulture

Fig. 7

From: The terpene synthase (TPS) gene family in kiwifruit shows high functional redundancy and a subset of TPS likely fulfil overlapping functions in fruit flavour, floral bouquet and defence

Fig. 7

Subcellular localisation of AcTPS in protoplasts. AcTPS-GFP translational fusion constructs were transiently expressed in N. benthamiana and analysed by confocal laser-scanning microscopy. GFP: GFP fluorescence; CA: chlorophyll auto-fluorescence from chloroplasts; R: fluorescence of red fluorescence protein from the vector (pMDC43) localised to the cytoplasm; brightfield: light microscopy images of intact mesophyll protoplasts; merged: visible and fluorescence signals (GFP + CA or GFP + R) combined. (A) AcNES3-GFP targeted to the cytoplasm. (B1) AcNES2-GFP targeted to the cytoplasm and (B2) to the chloroplast. (C) AcBCS-GFP targeted to the cytoplasm. (D) AcLIS/NES-GFP targeted to the chloroplast. (E) AcGES-GFP targeted to the chloroplast. Scale bars = 10 µm

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