Fig. 7

CmBBX7 and CmBBX8 do not mutually affect each other’s activity. Fluorescence images: from blue to red, the fluorescence value increases gradually. PC: positive control (AtARF5); NC: negative control (empty vector). b Vector construction diagrams for the reporter and effector constructs used in the transcription activity assay. The reporter construct contains five copies in tandem of the GAL4-binding sites upstream of the promoter (TATA), the firefly gene for luciferase, and Nos. The effector constructs contain the GAL4 DNA-binding domain (GAL4 DB) between the CaMV 35S promoter and Nos. c Relative LUC activity. Error bars indicate the standard deviations for six biological replicates. Significant differences are indicated with asterisks (**p < 0.01, ANOVA with Tukey’s post-hoc test)