Fig. 9
From: Salicylic acid-related ribosomal protein CaSLP improves drought and Pst.DC3000 tolerance in pepper
CaSLP enhances the binding of CaNAC035 to its target gene promoters. A Yeast two-hybrid assay of CaNAC035 and CaSLP. The full-length ones were CaNAC035 and CaSLP cloned into a pGBKT7 or a pGADT7 vector, respectively Yeasts grown in SD (-Trp/-Leu), SD (-Trp/-Leu/-His/-Ade) and SD (-Trp/-Leu/-His/-Ade + X-α-gal) media are indicated. B Bimolecular fluorescence complementation (BiFC) assay of CaNAC035 and CaSLP. A representative picture is shown here, GFP, green fluorescent protein. C Growth of yeast cells. D, F Diagrams of effector and reporter constructs. E luciferase (LUC)/Renilla luciferase (REN) activities detected from LUC/REN reporter system. G, H GUS activities assay the interaction of CaSLP and enhance the binding of CaNAC035 to the CaPR1 promoter. Values are means ± SD (n = 3 replicates). Asterisks indicate significant difference (*, P < 0.05; **, P < 0.01)