Fig. 7
From: Salicylic acid-related ribosomal protein CaSLP improves drought and Pst.DC3000 tolerance in pepper
Silencing of CaSLP by virus-induced gene silencing (VIGS) decreases Pst.DC3000 resistance in pepper. A, E Disease symptoms of CaSLP-silenced and control (TRV, tobacco rattle virus) plants before and after the Pst.DC3000 infection. Plants were infected with Pst.DC3000 for 3 days. B, F Histochemical staining with 3,3’-diaminobenzidine (DAB) and Trypan blue for analyzing the accumulation of H2O2 in the CaSLP-silenced and control plants before and after 3 d of Pst.DC3000 infection. C, G Trypan blue and DAB staining for cell death in the CaSLP-silenced and control plants before and after Pst.DC3000 infection. Bar = 50 μm. D-I Bacterial numbers (D), Chlorophyll content (H), and H2O2 content (I) of the CaSLP-silenced and control plants before and after 3 d of Pst.DC3000 infection. J-L The expression of the SA response genes of CaNPR1, CaABR1 and CaPR1. M, O2.− content of the CaSLP-silenced and control plants before and after Pst.DC3000 infection. Error bars represent ± SD (n = 3). Asterisks indicate a significant difference between CaSLP-silenced and control plants(*, P < 0.05; **, P < 0.01)