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Fig. 8 | Molecular Horticulture

Fig. 8

From: The NAC transcription factor MdNAC4 positively regulates nitrogen deficiency-induced leaf senescence by enhancing ABA biosynthesis in apple

Fig. 8

MdNAC4 interacts with MdPYL4. a Interaction between MdNAC4 and MdPYL4 in the Y2H assay. Full-length MdPYL4 was fused into the pGADT7 vector to obtain a recombinant plasmid (pGADT7-MdPYL4). The recombinant plasmid pGBKT7-MdNAC4 was obtained by fusing the MdNAC4 fragment without the autonomous activation domain into the pGBKT7 vector. Empty pGADT7 + pGBKT7-MdNAC4 (147-285 aa) and empty pGBKT7 + pGADT7-MdNAC4 were used as controls. The cotransformed yeast grown in SD (−T/−L), SD (−T/−L/−H/−A) and SD (−T/−L/−H/−A + X-α-gal) medium are indicated. b Interaction between the MdNAC4 and MdPYL4 proteins in the pull-down assay. The glutathione transferase (GST), MdPYL4-GST and MdNAC4-HIS proteins were induced by isopropyl thiogalactoside in Escherichia coli. The GST and MdPYL4-GST proteins were incubated with the MdNAC4-HIS protein, and the protein mixture was purified using the GST purification kit. c Subcellular localization of the MdPYL4 protein and the MdNAC4 protein. GFP, green fluorescent protein. d BiFC assays showed that the MdNAC4 protein interacted with the MdPYL4 protein. YFP, yellow fluorescent protein

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Molecular Horticulture

ISSN: 2730-9401

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