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Fig. 4 | Molecular Horticulture

Fig. 4

From: The NAC transcription factor MdNAC4 positively regulates nitrogen deficiency-induced leaf senescence by enhancing ABA biosynthesis in apple

Fig. 4

MdNAC4 caused early senescence induced by N deficiency in tobacco. a Leaf phenotypes of 4-week-old wild-type (Control) and transgenic tobacco (MdNAC4-L1, MdNAC4-L2 and MdNAC4-L3) grown in nitrate-deficient Hoagland nutrient solution for 3 weeks. Tobacco seedlings grown in Hoagland nutrient solution supplied with nitrate before treatment. Representative photographs were taken, where 1-12 represent the numbered leaf positions from the base to tip of tobacco leaves. b-e Total chlorophyll contents of tobacco leaves numbered 1-3, 4-6, 7-9, and 10-16 from 12 plants per indicated genotype. f-k Expression levels of NtNYC1, NtPAO, NtSGR1, NtSAG12, NtSAG29 and NtSAG39 in N-deficient wild-type and MdNAC4-overexpressing tobacco plants for 3 weeks. The expression level in the WT supplied with nitrate (+N) was set at 1. Error bars indicate the SDs of the three technical replicates and three biological replicates. Asterisks indicate significant differences between two independent samples according to t tests (*, P < 0.05 and **, P < 0.01)

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Molecular Horticulture

ISSN: 2730-9401

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