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Fig. 3 | Molecular Horticulture

Fig. 3

From: The NAC transcription factor MdNAC4 positively regulates nitrogen deficiency-induced leaf senescence by enhancing ABA biosynthesis in apple

Fig. 3

MdNAC4 activates the expression of MdNCED2. a Diagram of the MdNCED2 gene promoter region. P1-P4 represent the potential sites to which MdNAC4 might bind. b The electrophoretic mobility shift assay (EMSA) showed that the MdNAC4-GST fusion protein was bound to the MdNCED2 promoter. 5x, 50x and 100x represent the competitor concentrations. The unlabeled probes were used as competitors, with “Mut” representing the mutated probe in which the 5′-ACGTG-3′ motif was replaced by 5′-CCGTC-3′. c Structures of the reporter and effector vectors used in the dual-luciferase assays. The promoter fragment of MdNCED2 was fused into the pGreenII 0800-LUC vector to obtain the reporter plasmid. The MdNAC4 gene was fused to the pGreenII 62-SK vector to generate the effector plasmid. d Dual luciferase assays of tobacco leaves showed that MdNAC4 activated the expression of MdNCED2. e Relative LUC/REN activity analysis verified that MdNAC4 activated the expression of MdNCED2. Tobacco injected with empty vector was used as the control. Error bars indicate the SDs of the three technical replicates and three biological replicates. Different letters above the bars indicate significant differences according to one-way ANOVA (P < 0.05)

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