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Fig. 1 | Molecular Horticulture

Fig. 1

From: Melon (Cucumis melo) fruit-specific monoterpene synthase

Fig. 1

A Genomic organization of the MTPS cluster on chromosome 11. Filled box arrows indicate the positions of MTPS coding sequences. B Phylogenetic tree of MTPS. MTPS1, Melo3C023284; MTPS2, Melo3C023278; MTPS3, Melo3C023282; MTPS4, Melo3C023286; MTPS5, MELO3C023287; MTPS6, Melo3C023288; MTPS7, Melo3C023275; MTPS8, Melo3C023276; MTPS9, Melo3C034557; MsLS Mentha spica L-limonene synthase (GenBank accession number L13459); CuLS, Citrus unshiu d-limonene synthase (GenBank accession number BAD27257). C Heat map showing expression profile of MTPSs using RNA seq data (Yano et al. 2020). D Subcellular localization of MTPS7-GFP. Leaves of N. benthamiana were co-infused with Agrobacterium tumefaciens cells containing MTPS7-GFP plasmid and cells containing the 35S-P19 plasmid. Control plants were infused only with cells containing 35S-P19. Images were obtained three days after infiltration. E Selected-ion chromatogram (m/z = 92.5–93.5) of terpenoids produced by E. coli cells expressing MTPS isoforms. Each cell line was cultured in duplicates in the presence of 1/10 culture volume of dodecane. Terpenoids extracted in dodecane fraction were separated by GC–MS. Peaks corresponding to major terpenoids were labeled. The bottom panels show MS spectra for the identified peaks (blue) with the spectra of standards (red) in National Institute of Standards Mass Spectral Library

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