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Fig. 5 | Molecular Horticulture

Fig. 5

From: NtMYB12 requires for competition between flavonol and (pro)anthocyanin biosynthesis in Narcissus tazetta tepals

Fig. 5

Identification of candidate proteins NtWD40-1 and NtbHLH1 of the MBW complex. A coexpression network of NtMYB12 by WGCNA analysis; B NtMYB12 interacts with NtWD40-1 or NtbHLH-1 in yeast cells. Scale bars = 1 cm; C NtMYB12 colocalized with NtWD40-1 or NtbHLH-1 in the nucleus and cytoplasm of tobacco epidermal cells, Scale bars = 20 µm; D Scheme of various fragments of NtMYB12 used in bimolecular fluorescence complementation (BiFC) assay; E NtMYB12 interacts with NtWD40-1 or NtbHLH-1 in vivo by BiFC assay. Scale bars = 20 µm. F Coimmunoprecipitation detection of NtMYB12-HA with NtWD40-1-GFP and NtbHLH1-CFP or NtMYB12-HA with NtbHLH1-CFP by using antibody against HA and GFP for IP and immunodetection

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Molecular Horticulture

ISSN: 2730-9401

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