Fig. 4

NtMYB12 directly regulates NtFLS, NtLAR and NtDFR gene expression. A Scheme of constructs in dual luciferase activity assay; B luciferase fluoresce imaging and luciferase activity of cotransformed tobacco leaves with oeNtMYB12 and NtFLSpro:LUC relative to empty vector with NtFLSpro:LUC; C-D luciferase fluoresce imaging and luciferase activity of cotransformed tobacco leaves with oeNtMYB12 and NtDFRpro:LUC (C)or NtLARpro:LUC (D) relative to empty vector with NtFLSpro:LUC; E Scheme of constructs of various fragment NtFLSpro:LUC in dual luciferase activity assay; F luciferase fluoresce imaging and luciferase activity of cotransformed tobacco leaves with oeNtMYB12 and various fragment NtFLSpro:LUC relative to empty vector with NtFLSpro:LUC; G luciferase activity of cotransformed oeNtMYB12 with NtFLSpro:LUC or NtDFRpro:LUC or NtLARpro:LUC relative to empty vector with Pro:LUC in narcissus tepal protoplast; H luciferase activity of cotransformed oeNtMYB12 with various fragment NtFLSpro:LUC relative to empty vector with NtFLSpro:LUC in narcissus tepal protoplast; I Scheme of various promoter fragments of NtFLSpro, NtLARpro and NtDFRpro in ChIP-qPCR assay (J) Fold change enrichment normalized to INPUT of oeNtMYB12 on the promoters of NtFLS, NtDFR, NtLAR by ChIP. NtACTIN was used as an inner control, GFP alone was used as IP control. The error bar presented standard deviants of three biological replicates (n = 9). Asterisk presents the statistical significance compared to GFP plant (* p < 0.05; ** p < 0.01; ***p < 0.001)