Applications of CRISPR/Cas genome editing in economically important fruit crops: recent advances and future directions

Ma, Zhimin; Ma, Lijing; Zhou, Junhui

doi:10.1186/s43897-023-00049-0

Table 1 Recent advances of CRISPR/Cas genome editing in economically important fruit crops

From: Applications of CRISPR/Cas genome editing in economically important fruit crops: recent advances and future directions

Species	Promoter	Nuclease	Target Gene(s)	Target Trait(s)	Cas9 delivery method	Refence
Fragaria vesca	AtUBQ10	Cas9	TAA1, ARF8	Fruit development	Agrobacterium-mediated	(Zhou et al., 2018)
Fragaria vesca	35S	Cas9	YUCCA10	Fruit development	Agrobacterium-mediated	(Feng et al., 2019)
Fragaria vesca	35S	Cas9	SEP3	Flower and fruit development	Agrobacterium-mediated	(Pi et al., 2021)
Fragaria vesca	AtUBQ10	Cas9	AGL62, ALG80	Fruit development	Agrobacterium-mediated	(Guo et al., 2022)
Fragaria vesca	Ubi	nCas9 (D10A)	bZIPs1.1	Fruit sugar content	Agrobacterium-mediated	(Xing et al., 2020)
Fragaria vesca	Ubi	Cas9	MYB10, CHS, PDS, UF3GT, F3H, LDOX	Fruit coloration	Agrobacterium-mediated	(Xing et al., 2018)
Fragaria vesca	35S	Cas9	LAM	Plant architecture	Agrobacterium-mediated	(Feng et al., 2021a)
Fragaria ananassa	35S	Cas9	TM6	Flower and fruit development	Agrobacterium-mediated	(Martin-Pizarro et al., 2019)
Fragaria ananassa	35S	Cas9	RAP	Fruit coloration	Agrobacterium-mediated	(Gao et al., 2020)
Cucumis sativus	35S	Cas9	WIP1	Flower development	Agrobacterium-mediated	(Hu et al., 2017)
Cucumis sativus	35S	Cas9	SPT,ALC	Flower and fruit development	Agrobacterium-mediated	(Cheng et al., 2022)
Cucumis sativus	35S	Cas9	HEC2	Fruit development	Agrobacterium-mediated	(Wang et al., 2021e)
Cucumis sativus	35S	Cas9	NS	Fruit development	Agrobacterium-mediated	(Liu et al., 2022)
Citrullus lanatus	UBI	Cas9	PSK1	Resistance to Fusarium oxysporum	Agrobacterium-mediated	(Zhang et al., 2020b)
Citrullus lanatus	35S	Cas9	WIP1	Flower development	Agrobacterium-mediated	(Zhang et al., 2020a)
Citrullus lanatus	35S	Cas9	PDS	Albino phenotype	Agrobacterium-mediated	(Tian et al., 2017)
Citrullus lanatus	35S	Cas9	COMT1	Fruit quality, abiotic stress	Agrobacterium-mediated	(Chang et al., 2021)
Citrullus lanatus	35S	Cas9	NAC68	Fruit sugar content	Agrobacterium-mediated	(Wang et al., 2021b)
Citrullus lanatus	35S	Cas9	BG1	Seed development	Agrobacterium-mediated	(Wang et al., 2021c)
Cucumis melo	PcUbi4-2	Cas9	ROS1, CTR1-like	Fruit ripening	Agrobacterium-mediated	(Giordano et al., 2022)
Vitis vinifera	35S	Cas9	IdnDH	Fruit quality	Agrobacterium-mediated	(Ren et al., 2016)
Vitis vinifera	PcUbi4-2	Cas9	PDS	Albino phenotype	Agrobacterium-mediated	(Nakajima et al., 2017)
Vitis vinifera	VvUBQ2	Cas9	TMT1, TMT2, PDS	Fruit sugar accumulation; Albino phenotype	Agrobacterium-mediated	(Ren et al., 2021)
Vitis amurensis	35S	Cas9	PAT1	Cold response	Agrobacterium-mediated	(Wang et al., 2021d)
Vitis vinifera	35S	Cas9	CCD8	Shoot branching	Agrobacterium-mediated	(Ren et al., 2020)
Vitis vinifera	35S	Cas9	PR4b	Defense against the downy mildew	Agrobacterium-mediated	(Li et al., 2020a)
Vitis vinifera	35S	Cas9	MLO3, MLO4	Defense against the powdery mildew	Agrobacterium-mediated	(Wan et al., 2020)
Vitis vinifera	35S	Cas9	AGL104	Flower, fruit and seed development	Agrobacterium-mediated	(Sun et al., 2020b)
Citrus sinensis	35S	Cas9	PDS	Albino phenotype	Agrobacterium-mediated	(Jia and Wang, 2014)
Citrus sinensis	35S	Cas9	PDS	Albino phenotype	Agrobacterium-mediated	(Dutt et al., 2020)
Citrus sinensis	35S, Yao	LbCas12a	PDS, L0B1	Resistant to citrus canker	Agrobacterium-mediated	(Jia et al., 2019)
Citrus sinensis	35S	Cas9	NPR3	Systemic acquired resistance (SAR)	Cationic lipid transfection with or without PEG	(Mahmoud et al., 2022)
Citrus sinensis	CmYLCV	PC-ABE8e	LOB1	Resistant to canker	Agrobacterium-mediated	(Huang et al., 2022)
Citrus paradise	CmYLCV	nCas9	ALS	resistant to the herbicide	Agrobacterium-mediated	(Huang et al., 2022)
Malus prunifolia	35S	Cas9	PDS	Albino phenotype	Agrobacterium-mediated	(Nishitani et al., 2016)
Malus domestica	PcUbi4-2	nCas9	ALS, PDS	Resistance to chlorsulfuron and albino	Agrobacterium-mediated	(Malabarba et al., 2020)
Malus domestica	AtUBQ10	Cas9	DIPM4	Resistance to fire blight disease	Agrobacterium-mediated	(Pompili et al., 2020)
Malus domestica	AtUBQ10	LbCas12a	PDS	Albino phenotype	Agrobacterium-mediated	(Schropfer and Flachowsky, 2021)
Malus domestica	PcUbi4-2	Cas9	PDS, TFL1	Albino phenotype; early flowering	Agrobacterium-mediated	(Charrier et al., 2019)
Malus sieverii	ZmUbi, 35S	Cas9	PDS	Albino phenotype	Agrobacterium-mediated	(Zhang et al., 2021)
Malus domestica	35S	Cas9	CNGC2	Resistance to B. dothidea	Agrobacterium-mediated	(Zhou et al., 2020a)
Malus domestica	35S	Cas9	MKK9	Fruit color	Agrobacterium-mediated	(Sun et al., 2022)
Solanum lycopersicum	35S	Cas9	SHR	Root development	Agrobacterium-mediated	(Ron et al., 2014)
Solanum lycopersicum	35S	Cas9	AGO7	Leaf morphology	Agrobacterium-mediated	(Brooks et al., 2014)
Solanum lycopersicum	35S, AtUBQ	Cas9	PDS, PIF4	Albino phenotype, light signal transduction	Agrobacterium-mediated	(Pan et al., 2016)
Solanum lycopersicum	35S	Cas9	ARF4	Plant growth, resistance to abiotic stress	Agrobacterium-mediated	(Bouzroud et al., 2020)
Solanum lycopersicum	35S	Cas9	SRM1-like	Leaf development	Agrobacterium-mediated	(Tang et al., 2022)
Solanum lycopersicum	35S	nCas9 (D10A)	DELLA, ETR1	Hormone signaling	Agrobacterium-mediated	(Shimatani et al., 2017)
Solanum lycopersicum	35S	Cas9	SP, SP5G, ER	Plant architecture	Agrobacterium-mediated	(Kwon et al., 2020)
Solanum lycopersicum	35S	Cas9	BOP	Inflorescence development	Agrobacterium-mediated	(Xu et al., 2016)
Solanum lycopersicum	35S	Cas9	DOF9	Inflorescence and flower development	Agrobacterium-mediated	(Hu et al., 2022)
Solanum lycopersicum	Ubi	Cas9	ORRM4	Fruit ripening	Agrobacterium-mediated	(Yang et al., 2017)
Solanum lycopersicum	Ubi	Cas9	LncRNA1459	Fruit ripening	Agrobacterium-mediated	(Li et al., 2018a)
Solanum lycopersicum	35S, PcUbi4-2	Cas9	IAA9	Fruit development	Agrobacterium-mediated	(Ueta et al., 2017)
Solanum lycopersicum	PcUbi4-2	Cas9	GAD2, GAD3	Fruit quality	Agrobacterium-mediated	(Nonaka et al., 2017)
Solanum lycopersicum	35S	Cas9	PSY1	Fruit color	Agrobacterium-mediated	(Filler Hayut et al., 2017)
Solanum lycopersicum	Ubi	Cas9	MIR164A	Fruit ripening and chloroplast development	Agrobacterium-mediated	(Lin et al., 2022b)
Solanum lycopersicum	PcUbi4-2	Cas9	KIX9; SlKIX8	Plant organ size	Agrobacterium-mediated	(Swinnen et al., 2022)
Solanum lycopersicum	35S	Cas9	CRCa	Floral meristem determinacy	Agrobacterium-mediated	(Castaneda et al., 2022)
Solanum lycopersicum	35S	Cas9	CLV3, WOX9, TFL1,	Floral organ number, fruit size	Agrobacterium-mediated	(Rodriguez-Leal et al., 2017)
Solanum lycopersicum	35S	Cas9	ENO	Fruit size	Agrobacterium-mediated	(Yuste-Lisbona et al., 2020)
Solanum lycopersicum	Ubi	Cas9	MYC2	Plant development, disease resistance	Agrobacterium-mediated	(Shu et al., 2020)
Solanum lycopersicum	35S	Cas9	AGL6	Fruit development	Agrobacterium-mediated	(Klap et al., 2017)
Solanum lycopersicum	35S	Cas9	GGP1	Fruit quality	Agrobacterium-mediated	(Deslous et al., 2021)
Solanum lycopersicum	35S	Cas9	INVINH1,VPE5	Fruit sugar content	Agrobacterium-mediated	(Wang et al., 2021a)
Musa acuminata	35S	Cas9	RAS-PDS	Albino phenotype	Agrobacterium-mediated	(Kaur et al., 2018)
Musa acuminata	ZmUbi1, 35S	Cas9	PDS	Albino phenotype	Agrobacterium-mediated	(Naim et al., 2018)
Musa acuminata	Ubi	Cas9, LbCas12a	PDS	Albino phenotype	PEG mediated RNP	(Wu et al., 2020)
Musa acuminata	35S	Cas9	PDS	Albino phenotype	Agrobacterium-mediated	(Ntui et al., 2020)
Musa acuminata	35S	Cas9	LCYε	Fruit quality	Agrobacterium-mediated	(Kaur et al., 2020)
Musa acuminata	Ubi	Cas9	GA20OX2	Plant height	Agrobacterium-mediated	(Shao et al., 2020)
Musa acuminata	Ubi	Cas9	ACO1	Ethylene production; Fruit shelf	Agrobacterium-mediated	(Hu et al., 2021)
Musa acuminata	Ubi	Cas9	PDS	Albino phenotype	Protoplast transformation	(Zhang et al., 2022)
Musa acuminata	35S	Cas9	CCD4	Carotenoids metabolism	Particle bombardment	(Awasthi et al., 2022)
Actinidia chinensis	35S	Cas9	PDS	Albino phenotype	Agrobacterium method	(Wang et al., 2018b)
Actinidia chinensis	35S	Cas9	FLCL	Flower development	Agrobacterium method	(Voogd et al., 2022)
Actinidia chinensis	35S	Cas9	SyGl, CEN4	Flower development	Agrobacterium method	(Varkonyi-Gasic et al., 2021)
Actinidia chinensis	Ubi, 35S	Cas9	CEN, CEN4	Plant stature, early flowering	Agrobacterium method	(Varkonyi-Gasic, et al., 2019)

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