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Fig. 7 | Molecular Horticulture

Fig. 7

From: Warming-induced changes of broccoli head to cauliflower-like curd in Brassica oleracea are regulated by DNA methylation as revealed by methylome and transcriptome co-profiling

Fig. 7

Effects of temperature and DNA methylation inhibitor on DNA methylation levels and transcript levels of FCGs. A-B Tripartite relation among temperature, DNA methylation levels and transcript levels of some FCGs likely responsible for the 28 °C-induced floral cessation at the IM stage (A), and the 22 °C-induced floral cessation at the FP stage (B). A methylation level is the average of two biological replicates; and a transcript level represents the mean of a gene’s transcript levels present in three related transcriptomes. The green blocks indicate promoter region. Error bars indicate means ± SD. C-D qPCR analyses of randomly selected FCGs in mock and methylation inhibitor 5-azaC treated plants at the IM stage (C) and FP stage (D). Asterisks indicate significant differences relative to 16 °C (A, B), ANOVA analysis with Dunnett’s test (A) and T-test (B, C) were used. *p < 0.05, **p < 0.01, ***p < 0.001

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