Fig. 5

qPCR analyses of transcript levels of AtNAP, SAG202, and ICS1 in different mutants during senescence or after SA treatment. (a-c) Transcript levels of AtNAP, SAG202 and ICS1 in leaves of WT, atnap, sag202 and ics1 null mutants at different senescence stages (1, NS; 2, ES; 3, MS; 4, LS as described in legend to Fig. 1a). (d-f) Transcript levels of AtNAP, SAG202 and ICS1 in NS leaves of WT, atnap and sag202 null mutants at time points after treatment with 5 mM SA. Relative expression levels were calculated and normalized with respect to Actin 2 (ACT2) transcripts. Error bars indicate SD of three biological repeats. Significant (P < 0.05) differences between means are indicated by different letters using Tukey’s HSD test