Fig. 2

The amino acid mutated in SlSHN2 is located in a conserved middle motif of SHN. A Neighbour joining phylogenetic tree of SHN proteins from Arabidopsis thaliana (At), Solanum lycopersicum (Sl), Solanum pennellii (Sp) and Solanum tuberosum (St) species using MEGA11 (Tamura et al. 2021). AtSHN1, AT1G15360; AtSHN2, AT5G11190; AtSHN3, AT5G25390. SlSHN1, Solyc03g116610; SlSHN2, Solyc12g009490; SlSHN3, Solyc06g053240; SpSHN1, Sopen03g035520; SpSHN2, Sopen12g004490; SpSHN3, Sopen06g018310; StSHN1, Sotub03g030060; StSHN2, Sotub12g008480; StSHN3, Sotub06g017730. B Multiple sequence alignment of the characteristic conserved middle motif (termed ‘mm’) and C-terminal motif (termed ‘cm’) of SHN proteins using CLUSTALW (Larkin et al. 2007); 100% similar amino acids are indicated in pale green. The red box surrounds the conserved motif AKLRK. The mutated amino acid in the shn2 mutant (ΔSHN2) is indicated in red. C Comparison of the expression of SlSHN2 and SlSHN3 genes in the WT-like and shn2 mutant exocarp of 20 DPA fruit. Mean values of 3 biological replicates are given in FPKM with SD. b, P < 0.05 (Student’s t test)