Fig. 1

Mapping-by-sequencing identification of the shn2 mutation responsible for the glossy fruit phenotype. A Examples of fruit cuticle alterations from three independent mutant families. B diversity of micro-cracks, glossiness and colour of red ripe fruits from P7B8 cuticle mutant. C The wild-type (WT) parental line was crossed with the P7B8 glossy mutant. The BC1F2 progeny (216 plants) was screened for glossy fruits (approximately 25% of the plants) and WT-like fruits (approximately 75% of the plants) to constitute the WT-like and the glossy bulks (38 individuals each). D Allelic frequencies (y axis) of glossy and WT-like bulks are represented along tomato chromosomes (x axis) by green and orange lines, respectively. A sliding window of 10 SNPs was used. E, High scoring mutations identified on chromosome 12. Only one mutation (position 2,751,259 on chromosome 12) located in exonic region has a possible deleterious effect on the protein. F Fine mapping of the causal mutation using the BC₁F₂ population. Recombinant analysis of 768 BC₁F₂ individuals allowed us to locate the causal mutation at position 2,751,259 nucleotides on SL3.0 Heinz 1706 tomato genome sequence. Marker positions are indicated by grey triangles. Number of recombinants are shown below the position of the markers. Chromosomal constitution of the recombinants are represented by green and orange bars, for mutant and heterozygous segments, respectively. G, the single nucleotide transversion G to T at position 2,751,259 in the second exon of Solyc12g009490 leads to a missense mutation resulting in the K114N amino acid change