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Fig. 3 | Molecular Horticulture

Fig. 3

From: Yang cycle enzyme DEP1: its moonlighting functions in PSI and ROS production during leaf senescence

Fig. 3

Effect of MdDEP1 overexpression on the Yang’s cycle. A. Expression of pCaMV35S::MdDEP1-GFP in transiently transformed apple leaf protoplasts. All experiments were assayed 6–12 h after bombardment. Protoplasts expressing pCaMV35S::GFP were used as control. Bars = 50 μm. B. Immunoblot assay of protein extracts from apple leaf protoplasts, cytosol, chloroplast, and mitochondria, using specific antibodies against the chloroplast protein LHCB1, the mitochondria-specific cytochrome C protein, and the cytosol-localized protein ACTIN. C-E. Methionine contents (C), ethylene production (D), the expression of genes in Yang’s cycle (E) in the WT and three 35S::MdDEP1-Myc transgenic apple plantlets. F. WT and MdDEP1 overexpression plants treated with or without 150 mg L− 1 AVG for 2-weeks. G. Chlorophyll content of plants shown in (F). Note: In (C-E) and (G), the data are shown as the mean ± SE, which were analyzed based on more than 9 replicates. Statistical significance was determined using Student’s t-test in different samples. *P < 0.01; **P < 0.001

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