Fig. 4
From: A regulatory GhBPE-GhPRGL module maintains ray petal length in Gerbera hybrida
GhBPE binds to the GhPRGL promoter. A GhBPE binds to GhPRGL promoter according to the yeast one-hybrid results. The strain containing p53-AbAi/pGADT7-Rec-53 and pGhPRGL353-AbAi/pGADT7 vector served as the positive and negative controls, respectively. Black triangles indicate the fold-dilution of the bacterial concentration: 100, 10-1, and 10-2, from the left to right. B GhBPE binds to the GhPRGL promoter by electrophoretic mobility shift assay (EMSA). Binding of GhBPE and biotin-labeled GhPRGL probes is indicated by black arrows. The "+" and "–" symbols represent the presence and absence of the corresponding components, respectively. C Schematic diagram of the dual-luciferase assay vectors for GhBPE binding with GhPRGL promoter. D GhBPE binds to the GhPRGL promoter by dual-luciferase assay. Empty effector vectors were the controls. The LUC/REN fold-change value of the control was set to 1.0. Values are the mean ± SD of three biological replicates. Tukey’s HSD: * P < 0.05