Fig. 2

PyPIF5 protein suppresses PymiR156a expression by binding G-box in promoter. A The expression profile of PyPIF5. B Overexpression and suppression of PyPIF5. C Expression pattern analysis PymiR156a and PySPL9 in inoculated pear. D Dual-luciferase assay for the transcriptional activation of PyPIF5. E Electrophoretic mobility shift assay for examining the DNA-binding property of PyPIF5. As the progressively increase of Cold probe concentration, the accumulation of Free probe increases. The addition of mutant probes further confirmed that PyPIF5 can specifically bind to the G-box sequence within PymiR156a promoter