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Fig. 5 | Molecular Horticulture

Fig. 5

From: The leaf senescence-promoting transcription factor AtNAP activates its direct target gene CYTOKININ OXIDASE 3 to facilitate senescence processes by degrading cytokinins

Fig. 5

Physical interaction of AtNAP with the AtCKX3 promoter revealed by yeast one-hybrid assay. (A) Schematic representation of the constructs in the studies. The expression of prey GAD fused with or without (negative control or Ctr) AtNAP were driven by the GAL1 promoter (PGAL1). The LacZ gene driven by AtCKX3 promoter or its truncations or mutations served as the bait. (B) Activation of the LacZ reporter by binding of the fusion protein GAD-AtNAP to the AtCKX3 promoter. The AtCKX1 promoter lacked the motif and served as the other control. Blue color suggests a binding. The sequence in red represents the motif which AtNAP binds to. A of the translation start site was numbered as + 1. (C) Failure of AtNAP binding to the AtCKX3 promoter either containing transversion mutation or deletion of the motif sequence

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Molecular Horticulture

ISSN: 2730-9401

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