Skip to main content
Fig. 1 | Molecular Horticulture

Fig. 1

From: A cucumber NAM domain transcription factor promotes pistil development in Arabidopsis

Fig. 1

A Expression analysis of CsNAC2 in 1 mm buds of different genotype cucumber lines. Gynoecious line, G12 (CsACS1G/CsACS2); monoecy line, M12 (CsACS1/CsACS2); and hemaphrodite line, H34 (CsACS1G/csacs2). Error bars represent the SD from three biological replicates, and asterisks indicate significant differences in different treatments (t-test, ** p value < 0.01). B Spatial-temporal expression patterns of CsNAC2 in female development. Temporal expression patterns at different stages of female bud (1, 2, 5, and 10 mm buds were divided into 5 mm bud, 5 mm ovary, 10 mm bud, and 10-mm ovary, respectively) and spatial expression patterns of petal, stamen, stigma, mesocarp, and exocarp. Error bars represent the SD from three biological replicates. C In situ hybridization of CsNAC2 in gynoecy cucumber buds. Hybridization with antisense-probe in stage 7 (left), stage 8–2 (middle). Red arrows indicate hybridization signal. Hybridization with sense-probe presents in right. St: stamen, Pi: pistil. Bar = 200 μm. D Expression patterns of CsNAC2 in shoot apices of the chemical treatments and control cucumber lines. Eth: ethephon, AgNO3: silver nitrate, AVG: aminoethoxyvinyl-glycine. Error bars represent the SD from three biological replicates. Different letters (a, b) indicate significant differences (one-way ANOVA, p value < 0.01). E Expression level of CsNAC2 and CsACS2 in cucumber shoot apices of different concentrations treatment. Apical shoots of four-leaf stage seedlings were soaked in water containing 0, 0.1, 0.5, and 1.0 mM ethephon for 3 and 6 h, respectively. The circle indicates the expression of 10 biological replicates. The letters indicate the difference between each other p value < 0.01 in (a, b), (b, c) and (c, d) from t-test. F An unrooted phylogenetic tree of CsNAC2 from Arabidopsis and cucumber. The phylogenetic tree was constructed by the Neighbor-Joining method with 1000 bootstrap sampling. Bar represents patristic distances. G Subcellular localization of CsNAC2. Subcellular localization of CsNAC2-YFP fusion proteins in tobacco leaves. 35S:CFP indicates a marker for nuclear localization signal. H Phenotype result of Arabidopsis transgenic individual. Phenotypes of transgenic plants are T2 generation. All transgenic plants in different individual lines share the same phenotype, and only one line shows in result. I Floral organs phenotype of Arabidopsis transgenic individual. Flower bud phenotype of pAP3-vector and pAP3-CsNAC2 in stage 11(left) and flowering time (right). Bar = 0.2 cm. J The four types phenotypes of pAP3-CsNAC2 flower buds. Type I, normal looking but non-functional stamen, with papillar tissue on the tips. Type II, carpelloid stamen fuse with carpel. Type III, a carpelloid stamen not fuse with carpel. Type IV, no stamens and abnormal carpel. The white arrows indicate the abnormal floral organs. Bars = 0.2 cm. K Expression level of floral genes in transgenic Arabidopsis (left) and cucumber (right) plants. Error bars represent the SD from three biological replicates, and asterisks indicate significant differences in different treatments (t-test, ** p value < 0.01). L Ethylene production in pAP3-CsNAC2 the transgenic Arabidopsis plants. Error bars represent the SD from four biological replicates, and asterisks indicate significant differences in different treatments (Student’s t-test, ** p value < 0.01). M Expression level of ethylene related genes in the transgenic Arabidopsis. Error bars represent the SD from three biological replicates, and asterisks indicate significant differences in different transgenic plants (t-test, ** p value < 0.01). N Model of CsNAC2 functions during the development of female flowers in Arabidopsis and cucumber. The ethylene signal activated by the positive feedback loop induces the expression of CsNAC2. CsNAC2 then promotes female organ development via activates floral genes in an unknown way, and represses the ethylene related genes to avoid an excessive ethylene effect in the flower bud

Back to article page