Fig. 7

Transcriptional activity assay of SlDof1 in Nicotiana benthamiana leaves. (A) Diagram depicting the construction of the reporter and effector plasmids used in this assay. The reporter plasmids contain the promoters of putative SlDof1 target genes fused with firefly luciferase (LUC), with renilla luciferase (REN) driven by the CaMV35S promoter as an internal control. The SlDof1 coding sequence was cloned into the effector plasmid under the control of CaMV35S. The empty effector vector (35S-empty) was used as a negative control. The reporters and effectors were co-transformed into N. benthamiana leaves using the A. tumefaciens-mediated transformation method. OCST, OCS terminator. (B) Transcription activation or repression activity, which is expressed by the ratio of LUC to REN. Values are means ± SD of six independent experiments. Asterisks indicate statistically significant differences (P < 0.05; Student’s t-test). (C) A schematic summary of the mechanism by which SlDof1 regulates fruit ripening