Fig. 1

SlRUP negatively regulates the UVR8-mediated UV-B photomorphogenesis. (A) Transcript analysis of SlRUP in response to UV-B in Sluvr8, Slhy5 mutants compared with wildtype (AC, Ailsa Craig) by qRT-PCR. 6d old seedlings were irradiated with UV-B for different lengths. Mean and SE of three biological replicates are presented. (B) Representative seedlings of wildtype and Slrup mutants (CR8 and CR22), and SlRUP-GFP lines grown in white light or white light supplemented with UV-B. Bar = 1 cm. (C) and (D) Hypocotyl length of 6 d old seedlings grown under white light (WL) or white light supplemented with UV-B (UV-B), Mean and SE are presented (n = 30). (E) The hypocotyl length ratio of UV-B/WL. Asterisks indicate significant differences between transgenic lines and wildtype, differences with a P < 0.05 (*) and P < 0.01 (**) resulting from a one-way analysis of variance test. (F) UV-B induced anthocyanin accumulation. Mean and SE are presented (n = 3). (G) and (H) qRT-PCR analysis of SlHY5 and SlCHS1 in response to UV-B in wildtype, Slrup mutant, and SlRUP-GFP line for different UV-B radiation time. Mean and SE of three biological samples are presented. (I) SlRUP promotes redimeriation of SlUVR8 post UV-B treatment. 6 d old seedlings were irradiated with broadband UV-B for 0.5 h, followed by recovery in white light (WL) in the absence of UV-B for different time. Anti-SlUVR8 was used as the primary antibody for immunoblot analysis. Ponceau staining serves as loading control