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Fig. 5 | Molecular Horticulture

Fig. 5

From: PeNAC67-PeKAN2-PeSCL23 and B-class MADS-box transcription factors synergistically regulate the specialization process from petal to lip in Phalaenopsis equestris

Fig. 5

PeKAN2 expression during flower development and analyses of the role of PeKAN2. a Relative transcript levels of PeKAN2 in different organs of P. equestris var.trilip at different developmental stages(from stages S1 to S8 development). b Relative transcript levels of PeKAN2 in the petal and lip of P. equestris and relative transcript levels in the lip-like petal and lip of P. equestris var.trilip at S8 stage. c The phenotypes of PeKAN2-silenced lines. The red arrow represents the part where the mutation occurs after VIGS treatment, and the blue arrow represents the corresponding part in the Mock. d The epidermal cells of wild-type control (Left) and those of PeKAN2-silenced (right) flowers. Bar = 50 μm. e Relative transcript levels of PeKAN2, PeNAC67 and PeSCL23 in Mock and VIGS-treated lines. f Relative transcript levels of PeMADS3, PeMADS4 and PeMADS9 in Mock and VIGS-treated lines. g Yeast two hybrid assay of PeMADS3/PeMADS9 with PeKAN2. h BiFC assay was introduced to detect the interaction PeMADS3/PeMADS9 with PeKAN2 in tobacco leaves. Values are means ± SDs (n = 3). Asterisks in e and f indicate significant differences compared with the control, with one asterisk indicating P < 0.05 and two asterisks indicating P < 0.01

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Molecular Horticulture

ISSN: 2730-9401

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